RUNX1 anticorps

N° du produit ABIN350812

Cet anticorps Mouton Polyclonal détecte spécifiquement RUNX1 dans WB et IHC. Il présente une réactivité envers Humain.

Aperçu rapide pour RUNX1 anticorps (ABIN350812)

Antigène: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Reactivité: Humain

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp RUNX1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-RUNX1 anticorps

Fonction: Sheep antibody to Runx1

Specificité: Specific for Runx1.

Réactivité croisée: Humain, Souris, Rat

Réactivité croisée (Details): Other species not yet tested.

Purification: IgG

Immunogène: A synthetic peptide from human Runx1 conjugated to blue carrier protein was used as the antigen.

Isotype: IgG

Information d'application

Indications d'application: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Conseil sur la manipulation: Avoid freeze and thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Date de péremption: 12 months

Détails sur RUNX1

Antigène: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Autre désignation: Runx1

Sujet: FUNCTION: CBF binds to the core site 5'-PYGPYGGT-3' of a number of enhancers and promoters including murine leukemia virus polyomavirus enhancer T-cell receptor enhancers LCK IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation. SUBUNIT: Heterodimer with CBFB. RUNX1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Isoform AML-1L can neither bind DNA nor heterodimerize. Interacts with TLE1 and THOC4. Interacts with ELF1 ELF2 and SPI1. Interacts via its Runt domain with the ELF4 N-terminal region. Interaction with ELF2 isoform 2 (NERF-1a) may act to repress RUNX1-mediated transactivation. Interacts with MYST3 and MYST4. Interacts with SUV39H1 leading to abrogate the transactivating and DNA-binding properties of RUNX1. TISSUE SPECIFICITY: Highest levels in thymus bone marrow and peripheral blood.

UniProt: Q01196